Assessing the Nature of Human Brain-Derived Extracellular Vesicles on Synaptic Activity Via the Development of an Air-liquid Microfluidic Platform.

ABSTRACT: Brain-Derived Extracellular Vesicles (BDEVs) have been associated with important roles in functional neuron networks. However, the various models that have been used to study these roles fail to account for all the specificities of the human brain. This study presents a microfluidic platform capable of injecting and/or collecting BDEVs from Organotypic culture of Post-mortem Adult human Brain explants (OPAB) cultured at the air-liquid interface, while measuring electrical activity in real-time on 3D-microelectrode arrays (MEA). The platform design and custom-made program to control the system allows the automatic collection of BDEVs over days. Mass spectrometry analyses highlight that BDEVs are significantly enriched with synaptic proteins, such as Neural cell adhesion molecule, Syntaxin-1

SUBMITTER: Bernou C 

PROVIDER: S-EPMC12786325 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature