Non-Destructively Quantifying the Whole-Course Growth and Drug-Response of PDOs by an Automatic Microfluidic System Utilizing Chemiluminescence Detection.

ABSTRACT: Patient-derived organoids (PDOs) have become promising tools in precision medicine research. While conventional imaging techniques provide morphological assessment, they fail to reveal crucial molecular-level changes. Monitoring secreted biomarkers presents an alternative approach that can deliver real-time physiological data throughout the growth and drug response process. In this study, the non-destructive quantification for the whole-course growth and drug-response of PDOs is first realized using a multifunctional microfluidic chip-based system that integrates culturing, drug incubation, and biomarker detection. To validate the feasibility of this method, Carcinoembryonic Antigen (CEA), a broad biomarker, is selected to investigate its correlation with both organoid growth (over 6 days)

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12786359 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature