Transmission of Non-Constitutive Proteasomes Between Cells via Extracellular Vesicles.

ABSTRACT: Most intracellular proteins are degraded by the ubiquitin-proteasome system (UPS), with proteasomes directly hydrolyzing protein substrates. Specific forms of proteasomes (non-constitutive proteasomes), implicated in antigen presentation, cellular homeostasis maintenance and stress response have been described. However, proteasomes were also identified outside cells, where their function remains unclear. Proteasome secretion via extracellular vesicles (EVs) have been reported, though the direct transmission of non-constitutive proteasomes between cells has not been shown. Using genetically modified cells, including a human adenocarcinoma cell line SW620B8-mCherry expressing the β5i subunit of non-constitutive proteasomes fused to the mCherry protein, and a number of techniques, such as dif

SUBMITTER: Grigorieva EV 

PROVIDER: S-EPMC12786927 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature