Specific Glucagon Assay System Using a Receptor-Derived Glucagon-Binding Peptide Probe.

ABSTRACT: Glucagon is a peptide hormone secreted by pancreatic alpha cells which elevates blood glucose and plays a critical role in diabetes onset and homeostasis. The accurate assessment of glucagon concentration is challenging due to its structural similarity with other hormones, causing cross-reactivity in antibody-based methods. Rapid and specific glucagon detection is essential, particularly during hypoglycemia. This study aimed to develop glucagon-specific probes combining high specificity, rapid detection, and ease of operation. We designed novel peptide-based probes by screening glucagon-binding peptides from the glucagon receptor sequence using a peptide array method. This strategy, based on receptor amino acid sequences, can be applied to the identification of binding peptides for other h

SUBMITTER: Shigeto H 

PROVIDER: S-EPMC12786957 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature