Reactivity of Antibodies Immobilized on Gold Nanoparticles: Fluorescence Quenching Study.

ABSTRACT: Antibodies labeled with gold nanoparticles are widely used in analytical systems. Consequently, the choice of methods for producing such conjugates requires a precise determination of the retained reactivity of the antibodies. Existing methods give highly variable results, necessitating new, simple, and accurate approaches. This study demonstrates how a fluorescein (FL) and anti-FL antibody pair can be used to comparatively evaluate conjugation conditions. The method is based on the quenching of fluorescein emission after binding to antibodies, enabling real-time monitoring of interactions in the reaction medium. Using this approach, we compared a series of conjugates of gold nanoparticles (average diameter 24 nm) and anti-FL antibodies that were obtained with antibody concentrations of 5,

SUBMITTER: Sotnikov DV 

PROVIDER: S-EPMC12787391 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature