LcSHMT4 from Sheepgrass Improves Tolerance to Cadmium and Manganese and Enhances Cd and Mn Accumulation in Grains.

ABSTRACT: Heavy metal contamination is a serious environmental problem worldwide, with substantial negative ecological and economic effects. Serine hydroxymethyltransferase (SHMT) is a key metabolic and photorespiratory enzyme in plant cells, and it is also involved in stress responses. In this study, LcSHMT4 was isolated from sheepgrass (Leymus chinensis (Trin.) Tzvel) after transcriptome sequence analysis. The transcript levels of LcSHMT4 in sheepgrass seedlings increased under Cd and Mn stresses, and subcellular localization analysis in tobacco leaves revealed that its encoded protein localizes at the mitochondria. Transgenic yeast and rice lines overexpressing LcSHMT4 showed increased tolerance to Cd and Mn, compared with that of their controls. In addition, compared

SUBMITTER: Wang J 

PROVIDER: S-EPMC12787419 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature