Genomic Identification and Biochemical Characterization of Methyl Jasmonate (MJ)-Inducible Terpene Synthase Genes in Lettuce (<i>Lactuca sativa</i> L. cv. Salinas).

ABSTRACT: Terpenes are diverse plant metabolites with essential ecological and physiological functions, yet their biosynthetic regulation in lettuce (Lactuca sativa L.) remains poorly understood. By integrating volatile profiling, genome-wide identification, and biochemical characterization of terpene synthase (TPS) genes, we elucidated how methyl jasmonate (MJ) induces terpene formation in lettuce seedlings. Headspace analysis of 10-day-old seedlings revealed that while mock-treated tissues emitted no detectable volatiles, MJ elicitation triggered the de novo production of a terpene blend dominated by (E)-β-ocimene (9.3-14.6%), (E)-β-caryophyllene (37.2-46.9%), and caryophyllene oxide (26.2-41.4%). A genome-wide search identified 54 putative LsTPS genes, often clustered with pr

SUBMITTER: Singh A 

PROVIDER: S-EPMC12787478 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature