3D organotypic skin models recapitulate autoantibody-driven pemphigus pathomechanisms and targeted therapeutic response.

ABSTRACT: Advanced three-dimensional (3D) tissue models that recapitulate autoimmune disease progression can enable mechanistic studies and accelerate the development of targeted therapies with reduced reliance on systemic immunosuppression. Here, we present a dynamic biofabrication strategy combining 3D bioprinting and cell self-organization to create multilayered skin constructs with a stratified epidermis atop a vascularized, fibroblast-remodeled dermis. This platform reconstitutes native skin architecture to model pemphigus vulgaris, an autoantibody-driven blistering disorder. Tightly reassembled keratinocytes form cell-cell adhesions that replicate pathogenic antibody-induced disruption of the epidermal barrier, while embedded vasculature and fibroblasts shape dermal barriers that regulate mole

SUBMITTER: Zhai H 

PROVIDER: S-EPMC12787515 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature