Biogenic Quorum-Sensing Amides from <i>Streptomyces</i> sp. NP10.

ABSTRACT: Volatile organic compounds produced by microbes are increasingly recognized as modulators of microbial interactions and mediators of both intra- and inter-kingdom communication. This study explored the possible ecophysiological roles of nine amides from Streptomyces sp. NP10 in quorum sensing (QS) and biofilm formation in Pseudomonas aeruginosa PAO1. GC-MS profiling, synthesis, spectral validation, and co-injection experiments confirmed compound identities. Notably, N-(3-methyl-2-butenyl)acetamide is reported as a new natural product and N-(2-methylbutyl)acetamide as a new Streptomyces-produced metabolite. At subinhibitory concentrations (250 μg/mL), most of the amides enhanced P. aeruginosa biofilm formation, with N-(2-methylbutyl)acetamide

SUBMITTER: Gencic MS 

PROVIDER: S-EPMC12787655 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature