Recombinant G-CSF-ApoAI Fusion Protein Is a Pleiotropic Factor.

ABSTRACT: In this study, we report the development of a recombinant human G-CSF fused with apolipoprotein A-I. The chimeric protein was expressed in Pichia pastoris. Using human bone marrow cells, the fusion protein was shown to retain the granulocyte activity of authentic G-CSF, more effectively inducing the differentiation and maturation of segmented neutrophils and maintaining the viability of progenitor cells. Using human mononuclear cells and THP cells, the resulting protein demonstrated monocytic activity, manifested by an increase in both total and CD14+ cell counts. By maintaining cell viability, the chimeric protein reduced the number of cells expressing caspase 3/7. G-CSF-ApoAI demonstrated accelerated cytokine regulation, promoting a more rapid transition of inflammation phases, ac

SUBMITTER: Miroshnichenko S 

PROVIDER: S-EPMC12787894 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature