Novel Method for Characterizing Humic Substances Using Fluorescent Solvatochromism.

ABSTRACT: Charge-transfer-type fluorochromes, which exhibit shifts in fluorescence intensity and emission wavelength in response to solvent polarity changes, have been widely employed to investigate solute-solvent interactions. Humic substances (HSs) are naturally occurring macromolecular organic acids derived from plant residues, with structural properties that vary depending on their origin and environmental conditions. The polarity of HSs is closely associated with the mobility and toxicity of environmental pollutants, making their chemical characterization essential. In this study, we developed a rapid and straightforward method to characterize HS polarity using fluorescent solvatochromism. The fluorescence peak shifts of four dyes-8-anilino-1-naphthalenesulfonic acid (ANS), acridine orange (AO)

SUBMITTER: Sazawa K 

PROVIDER: S-EPMC12787976 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature