Establishment of ITS-Derived Species-Specific PCR Assay Method for Discriminating Herbal Medicine Descurainiae Semen from Its Commercial Adulterants.

ABSTRACT: Accurate authentication of herbal medicine Descurainiae Semen, the tiny seeds of Descurainia sophia, is challenging due to their morphological similarity to various adulterants. To develop a precise and reliable molecular identification method, we conducted comparative analyses of rDNA-ITS sequences using D. sophia and five adulterant species and subsequently developed species-specific sequence-characterized amplified region (SCAR) markers. The discriminatory power and detection limits of these markers were evaluated using serially diluted genomic DNA from each species and commercially available Descurainiae Semen, respectively. The SCAR markers developed in this study enabled the detection of adulterant contamination at levels as low as 0.01-1%. Among several potential adult

SUBMITTER: Kim WJ 

PROVIDER: S-EPMC12788001 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature