A 3D Microfluidic Paper-Based Analytical Device with Smartphone-Based Colorimetric Readout for Phosphate Sensing.

ABSTRACT: In this work, a 3D microfluidic paper-based analytical device (3D-µPAD) was developed for the smartphone-based colorimetric determination of phosphate in environmental samples. The assay relied on the formation of a blue-colored product (molybdenum blue) in the detection area of the 3D-µPAD upon reduction of the heteropolyacid H₃PMo₁₂O₄₀ formed in the presence of phosphate. A number of experimental parameters were optimized, including geometric aspects of 3D-µPADs, digitization and image processing conditions, the amount of chemicals deposited in specific areas of the 3D-µPAD, and the reaction time. In addition, the stability of the device was evaluated at three different storage temperatures. Under optimal conditions, the working range was found to be from

SUBMITTER: Grana-Dosantos JM 

PROVIDER: S-EPMC12788290 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature