Comprehensive transcriptome and metabolome analysis to exploration the effects of TCs on GCs lipid metabolism at goose pre-ovulatory follicle.

ABSTRACT: Granulosa cells (GCs) and theca cells (TCs) have complex communication and interactions. Lipid metabolism in GCs is important for follicle development, which is also inseparable from the role of TCs; however, the underlying mechanisms remain unclear. In this study, we selected the pre-ovulatory (F1) follicle of geese-a poultry species with relatively low egg production-which exhibits high lipid content and vigorous lipid metabolism. Using transcriptomic and metabolomic approaches, we analyzed the effects of TCs on GCs in a co-culture model. We identified and screened the core functions and signaling pathways associated with the differentially expressed metabolites (DEMs) and genes (DEGs) between the mono-culture and co-culture groups. Key metabolites and genes within the core pathway were

SUBMITTER: Ma J 

PROVIDER: S-EPMC12788666 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature