Characterization of extended-spectrum cephalosporin-resistant Klebsiella recovered from dairy manure in Southern Ontario, Canada.

ABSTRACT: Extended-spectrum cephalosporin (ESC)-resistant Klebsiella pneumoniae are a problem in human patients and have been studied extensively. However, there is a paucity of information regarding ESC-resistant K. pneumoniae from livestock in general, and in Canada in particular. This study characterized ESC-resistant K. pneumoniae recovered from dairy manure in Ontario, Canada, and their ESC-resistance plasmids. ESC-resistant K. pneumoniae (n = 73) and K. quasipneumoniae (n = 11) isolates were screened by PCR for blaCTX-M, blaCMY and blaSHV prior to undergoing antimicrobial susceptibility testing using disk diffusion. Isolates from dairy manure carrying blaCTX-M (n = 74), and additional isolates from turkeys (n = 8) and dogs (n = 2), underwent short-read whole genome sequencing (WGS) and a subse

SUBMITTER: Anderson REV 

PROVIDER: S-EPMC12788680 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature