Beyond colonoscopy, faecal DNA mutation screening provides a potential and viable path to early colorectal cancer detection.

ABSTRACT: Colorectal cancer (CRC) remains a leading cause of cancer-related morbidity and mortality worldwide, with early detection critical for improving clinical outcomes. In Denmark's CRC screening program, nearly 60% of patients undergoing colonoscopy do not have polyps requiring treatment, highlighting the need for more sensitive and less invasive screening methods to improve early detection and reduce unnecessary procedures. In this study, we assessed the potential of two faecal DNA-based methods for detecting CRC-related mutations. Extracted DNA was analysed using next-generation sequencing (NGS) and digital PCR (dPCR). NGS targeted a gene panel covering the most frequently mutated sites in CRC, while dPCR focused on six specific CRC-associated mutations. A common limitation across both metho

SUBMITTER: Alfaro-Nunez A 

PROVIDER: S-EPMC12789060 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature