Gut-ovary axis and multiomic insights into PCOS in a DHEA-induced rat model.

ABSTRACT: This study aims to investigate gut microbiota and metabolomes of fecal, cecum, serum, and ovarian tissues in a DHEA-induced rat polycystic ovary syndrome (PCOS) model and explore the gut-ovary axis in PCOS pathogenesis. Rats were treated with DHEA or sesame oil to create PCOS and Control groups. The PCOS model was confirmed by ovarian morphology, estrous cycle, and serum hormone levels (FSH, LH, and E2). Stool, serum, cecum, and ovarian tissues underwent untargeted metabolomic analysis, and gut microbiota were assessed using 16S rRNA sequencing. Differentially expressed metabolites (DEMs) were analyzed using KEGG function and pathway enrichment analyses. Pearson's correlation analysis between DEMs and gut microbiota was performed. RT-PCR and Western blotting analyses determined intestinal

SUBMITTER: Chen Z 

PROVIDER: S-EPMC12789612 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature