Trichosanthin elicits antitumor activity via MICU3 mediated mitochondria calcium influx.

ABSTRACT:

Introduction

Trichosanthin (TK) is a glycoprotein extracted from the Chinese medicinal herb Trichosanthes kirilowi, which has anti-virus and anti-tumor activity. However, the target and detailed mechanism of TK remains elusive.

Objectives

We aimed to identify novel antitumor targets of TK in lung adenocarcinoma and study its anti-tumor mechanism.

Methods

We utilized a Lewis lung carcinoma mouse model to evaluate the inhibition of TK on tumor growth. CCK8 assay was utilized to calculate IC₅₀ of trichosanthin on A549 and H1299. In-vitro cellular assays and in-vivo xenograft mice studies were used to investigate MICU3 overexpression and TK treatment on tumor growth. Fluo-4 dye and JC-1 staining was used to measure the mitochondrial calcium levels and membrane

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12789729 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature