Exerkine irisin mitigates cognitive impairment by suppressing gut-brain axis-mediated inflammation.

ABSTRACT:

Introduction

Exercise has been recognized to improve cognitive performance by optimizing gut flora and up-regulating exerkine irisin.

Objective

Although exercise-induced irisin is beneficial to cognitive improvement, whether this benefit is achieved by optimizing gut microbiota and metabolites is not fully explored.

Methods

After aerobic exercise and exogenous irisin interventions for 12 weeks, the 16S rRNA and metabolites in feces of 21-month-old mice were analyzed. Meanwhile, the differential miRNAs and mRNAs in hippocampal tissues were screened by high-throughput sequencing. Relevant mRNAs and proteins were evaluated by RT-PCR, Western blot, and immunofluorescence.

Results

Compared with the young control mice, irisin levels and cognitive capacity of aged mic

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12789735 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature