Fibroblast Dysfunction and Immune Dysregulation Impair Palatal Wound Healing in Diabetic Rats.

ABSTRACT:

Objective

This study aimed to investigate how diabetes mellitus affects palatal wound healing, focusing on inflammatory responses, gene expression dynamics, and fibroblast function.

Methods

A standardized full-thickness palatal wound model was established in streptozotocin-induced diabetic and non-diabetic rats. Wound healing progression was assessed via gross observation, histological analysis, and immunohistochemistry (CD3, IBA1) at postoperative days (PID) 0, 3, 7, and 14. Wound-edge tissues were collected at PID 0, 3, and 7 for RNA sequencing. The resulting transcriptomic data were analysed using gene set enrichment analysis (GSEA) and weighted gene co-expression network analysis (WGCNA). Primary palatal fibroblasts were isolated from both groups and subjected to in vitr

SUBMITTER: Pan L 

PROVIDER: S-EPMC12790083 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature