Evaluating the osteoclastogenic potential of peripheral blood mononuclear cells in children with chronic nonbacterial osteomyelitis.

ABSTRACT: Chronic nonbacterial osteomyelitis (CNO) is an autoinflammatory bone disease in which osteoclastogenesis may play a critical role. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) has been shown to inhibit osteoclastogenesis derived from PBMCs. Our objectives were to determine whether osteoclastogenesis is altered (1) in patient PBMCs, (2) by patient serum, and (3) by a CTLA-4 analog, abatacept. Patients with active or inactive CNO, inactive juvenile idiopathic arthritis (JIA), and healthy controls were enrolled. CD14+ monocytes isolated from healthy donors were incubated with serum samples, macrophage colony-stimulating factor (MCSF), and RANKL for 7 d. Tartrate-resistant acid phosphatase positive cells with 3 or more nuclei were counted and compared across samples. In another study,

SUBMITTER: Bui JP 

PROVIDER: S-EPMC12790276 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature