An optimized ¹³C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins.

ABSTRACT: Biomolecular dynamics in the microsecond-to-millisecond (µs-ms) timescale are linked to various biological functions, such as enzyme catalysis, allosteric regulation, and ligand recognition. In solution state NMR, Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments are commonly used to probe µs-ms timescale motions, providing detailed kinetic, thermodynamic, and mechanistic information at the atomic level. For investigating conformational dynamics in high-molecular-weight biomolecules, methyl groups serve as ideal probes due to their favorable relaxation properties, and ¹³C CPMG relaxation dispersion is widely employed for characterizing dynamics in selectively ¹³CH₃-labeled samples. However, conventional schemes that apply CPMG pulses with c

SUBMITTER: Yuwen T 

PROVIDER: S-EPMC12790411 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature