Harziachalasins A-G, polycyclic-fused cytochalasins from the endophytic fungus Trichoderma harzianum MLJ-4 with HIV latency reversal activity.

ABSTRACT: Seven new polycyclic-fused cytochalasins (CYTs), harziachalasins A-G (1-7), together with three known analogues (8-10) were isolated from the solid culture of the endophytic fungus Trichoderma harzianum MLJ-4, which was originally isolated from the leaves of Asclepias curassavica. The planar and absolute structures of all new compounds were determined on the basis of extensive spectroscopic data (1D, 2D NMR and HR-ESI-MS), NMR calculations with DP4 + probability analysis, and theoretical simulations of ECD spectra. Compound 1 represents the first example of 5/6/6 tricyclic CYT featuring a 2-methyl-4-oxopentyl side chain at the C-14 position. This novel architecture originates from a 5/6/6/7 tetracyclic CYT precursor through sequential oxidative cleavage of the C-19-C-20 bond followed by de

SUBMITTER: Zhang YJ 

PROVIDER: S-EPMC12790558 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature