Mapping of PTP1B, TCPTP, SHP2, and Putative Substrates Reveals Novel Networks in Glomerular Podocytes.

ABSTRACT: Preservation of the insulin-sensitive glomerular podocyte is imperative for normal kidney function. The protein tyrosine phosphatases (PTPs), protein tyrosine phosphatase 1B (PTP1B), T-cell protein tyrosine phosphatase (TCPTP), and Src homology phosphatase 2 (SHP2) are established regulators of insulin signaling in vivo and implicated in renal function. However, knowledge gaps exist regarding the roles of these enzymes and their integrated modulation of signaling in podocytes. Accordingly, uncovering the mediators of PTP function is critical to elucidate their modes of action and help develop mechanism-based interventions for podocytopathies. We generated E11 podocyte cell lines expressing the substrate-trapping mutants of these PTPs and then used immunoprecipitation and mass spectrometry

SUBMITTER: LeBleu G 

PROVIDER: S-EPMC12790674 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature