Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.

ABSTRACT: Fungi are a diverse group of eukaryotic organisms, with over 1.5 million species inhabiting ecosystems worldwide. Many fungi grow as filaments (hyphae) and play critical ecological roles, both beneficial and harmful. Understanding their functions often requires generating knockout mutants and performing comparative analyses with wild-type strains. However, traditional methods for screening knockout mutants are labor-intensive, time-consuming, and limit the rapid identification of successful transformants. Here, we present a high-throughput droplet microfluidics platform capable of screening and sorting fungal transformants at single-cell resolution, significantly improving efficiency compared to conventional methods. The workflow involves encapsulating individual fungal transformants in pi

SUBMITTER: Li Y 

PROVIDER: S-EPMC12790921 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature