Insulin-like growth factor binding protein 7 identified in aged dental pulp by single-cell RNA sequencing.

ABSTRACT:

Introduction

Aging influences the regenerative and reparative functions of dental pulp, and an in-depth and complete understanding of aged dental pulp is highly important.

Objective

This study aimed to explore the heterogeneity of young and aged dental pulp tissue via single-cell RNA sequencing (scRNA-seq), search novel markers of aged dental pulp, and further explore their mechanism.

Methods

ScRNA-seq was employed to analyze the heterogeneity of young and aged dental pulp tissue, and immunohistochemical staining was used to detect new marker Insulin-like Growth Factor Binding Protein 7 (IGFBP7) in aged dental pulp. Differentially expressed genes (DEGs) between young and aged dental pulp tissue related with senescence-associated secretory phenotype (SASP) were validat

SUBMITTER: Tong Z 

PROVIDER: S-EPMC12793749 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature