Hydrogen-bond-assisted meta-nitrogen-doped graphyne enables real-time electrocatalytic NADH tracking in single cells.

ABSTRACT: Nicotinamide adenine dinucleotide (NADH), a crucial biomarker for cellular redox homeostasis, is intrinsically linked to mitochondrial function and neurodegenerative diseases. However, electrochemical detection of intracellular NADH faces dual challenges: low abundance and biofouling, as well as high overpotential. Herein, we designed a hydrogen-bond-assisted meta-nitrogen/oxygen co-doped graphyne (3NGYO) nanoelectrode for high-performance NADH sensing. Precise tri-N doping in graphyne (3NGY) generates sp²-N atoms associated with hydrogen atoms. Specifically, pyrrolic N-H forms a 2.502 Å N-H⋯O[double bond, length as m-dash]P hydrogen bond with NADH's PO₄ groups, boosting adsorption energy to -5.48 eV and reducing NADH oxidation potential to 0 V. This achieves a

SUBMITTER: Wei J 

PROVIDER: S-EPMC12794285 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature