Inhibition of Wnt signaling in primary human hepatocytes promotes Plasmodium falciparum liver stage development.

ABSTRACT: After infection of the human host, the first stage of the Plasmodium falciparum (Pf) lifecycle takes place in the liver. Understanding of host-parasite interactions during liver stage development is compromised by the rapid loss of functionality and Pf permissiveness of cultured primary human hepatocytes (PHHs). Here, we substantially delay the loss of Pf permissiveness by using a medium containing serum-replacement and signal transduction inhibitors. We analyzed and integrated transcriptomic profiles of cultured PHHs with the phenotypic presentation of developing Pf liver stages, revealing a number of host signaling pathways that contributed to dedifferentiation of hepatocytes and influenced Pf liver stage development. In particular, inhibition of the Wnt pathway showed a significant posi

SUBMITTER: Kanyal A 

PROVIDER: S-EPMC12795456 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature