Patient-derived organoids and neurospheres recapitulate salient features of primary tumor heterogeneity.

ABSTRACT:

Background

The intra- and inter-tumoral heterogeneity of glioblastoma (GBM) represents a significant therapeutic challenge and barrier to the generation of reliable and accurate models for in vitro studies. Three-dimensional models, such as patient-derived neurospheres (PDN) and organoids (PDO), recapitulate complex cellular states. However, the direct comparison of models derived in parallel from the same primary tumor tissue has never been performed. The aim of this study was to determine the tumor cell composition of PDN and PDO models relative to matched primary GBM tissue.

Methods

Four GBM surgical samples were used to establish matched PDO and PDN models, which were genomically verified using single nucleotide polymorphism array. DNA methylation, histology, and

SUBMITTER: Moore Z 

PROVIDER: S-EPMC12795600 | biostudies-literature | 2026 Jan-Dec

REPOSITORIES: biostudies-literature