Phylogenetic and structural analyses reveal Cdc2-like kinases (CLKs) as ancient regulators of thermosensitive splicing.

ABSTRACT: The family of Cdc2-like kinases (CLKs) plays a critical role in regulating both constitutive and alternative pre-mRNA splicing. Of particular interest, CLKs exhibit unique thermosensitive properties characterized by increased activity at lower physiological temperatures. In this study, we integrate phylogenetic, protein-interaction, and structural analyses to investigate the evolutionary history and functional adaptation of CLKs across protista, fungi, plants, and metazoans. Our phylogenetic analysis, comprising 149 CLK homologs from 86 species, traces this gene back to the last eukaryotic common ancestor. The results reveal lineage-specific patterns of gene duplication and loss, including complete loss of CLKs in seven protist lineages and in microsporidian fungi. Interolog mapping identi

SUBMITTER: Ogle RA 

PROVIDER: S-EPMC12795684 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature