Investigating the Therapeutic Efficacy of Quality-Controlled, miR-146a-5p-Enriched Small Extracellular Vesicles Derived From MSCs Against Idiopathic Pulmonary Fibrosis.

ABSTRACT: Small extracellular vesicles derived from umbilical cord mesenchymal stem cells (UC-sEvs) may be used for the treatment of idiopathic pulmonary fibrosis (IPF) because of their ability to control inflammation and inhibit fibrosis. However, the lack of clarity regarding the treatment mechanism of IPF and the corresponding quality standards limit the clinical application of these small extracellular vesicles. Here, we established a good manufacturing practice (GMP) grade process for isolating UC-sEvs, and RNA-seq was performed to screen for potential therapeutic cargo in the product to confirm the therapeutic effect of nebulized UC-sEv agents against IPF. Functionally, UC-sEvs inhibited the pulmonary inflammatory response by regulating macrophage function, thereby suppressing the bleomycin to

SUBMITTER: Wang X 

PROVIDER: S-EPMC12795917 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature