NS2B is indispensable for NS3/NS5 interaction of Tembusu virus in living cells.

ABSTRACT: Tembusu virus (TMUV) causes egg-drop syndrome and neurological signs in duck, goose and chicken, posing a great threat to the waterfowl industry. In the present study, we established an in vivo protein-protein interaction system for the NS2B-3 and NS5, together with ​co-immunoprecipitation (Co-IP)​, we demonstrated that NS2B3 (polyprotein) directly binds NS5 in live cells, while NS3 alone fails to interact with NS5-indicating NS2B is indispensable for NS3/NS5 binding. Mutations disrupting NS3's protease active site (to abrogate NS2B3 self-cleavage) or truncations of NS2B both abolished NS2B3/NS5 interactions, highlighting the structural integrity of NS2B-including its transmembrane domains (TMDs) is essential for stabilizing NS3's overall conformation, or cellular localization and facilita

SUBMITTER: He Y 

PROVIDER: S-EPMC12796533 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature