Metabolic engineering enables <i>Escherichia coli</i> to grow on 1,3-propanediol.

ABSTRACT: 1,3-propanediol (1,3-PDO) is used to synthesize plastics used in many consumer products. As the demand and production of such plastics increase, a technology will be needed to utilize 1,3-PDO released from the plastics after their disposal. In our previous study, we developed the strain (BA07Δ) that could use malonate semialdehyde (MSA, an important intermediate in the 1,3-PDO assimilation pathway) as the major carbon source. Here, we present construction of PA16, a strain which could grow to an OD₆₀₀ of 7 by consuming 6.5 g/L of 1,3-PDO within 72 h in M9-based medium supplemented with 1 g/L of complete supplement mixture (CSM). This was achieved by adaptive laboratory evolution (ALE) after extending the pathway in BA07Δ through the introduction of a 1,3-pro

SUBMITTER: Poon NY 

PROVIDER: S-EPMC12796543 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature