Long non-coding RNA UCA1 modulates SMARCA2-containing SWI/SNF chromatin remodeling complexes in human colorectal cancer.

ABSTRACT: Differential long non-coding RNA (lncRNA) expression has been correlated to clinical characteristics of colorectal cancers (CRCs), which are the second leading cause of cancer-related deaths. LncRNA UCA1 plays a role in epigenetic gene regulation in diverse cancers. We studied CRC cell properties in CRISPR-Cas9 HT29-derived models and, interestingly, UCA1-depleted HT29 cells presented an increased stem-cell phenotype. We show that loss of UCA1 modulated SWI/SNF chromatin remodeling complexes, previously shown to be involved in maintaining stem-cell properties. Not only was UCA1 permissive for induced SWI/SNF subunit SMARCA2 (BRM) expression upon chemo-drug treatment, but it also affected subunit compositions of SWI/SNF complexes by direct interaction of UCA1 with both ATP helicase BRM and

SUBMITTER: Neve B 

PROVIDER: S-EPMC12796597 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature