HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription.

ABSTRACT: The High Mobility Group Nucleosome-binding (HMGN) proteins are small, abundant nuclear proteins that directly bind nucleosomes and form a major component of chromatin. HMGN proteins localize to enhancers and actively transcribed genes across the genome; however, their roles in regulating chromatin structure and transcription remain poorly understood. Although it is well established that HMGN proteins bind to the H2A-H2B acidic patch on nucleosomes, other potential nucleosome targeting mechanisms, including histone post-translational modifications and histone variants, remain unclear. To investigate the nucleosomal binding preferences and function of HMGN proteins, we engineered mouse embryonic stem cells (mESCs) lacking HMGN1 and/or HMGN2 (Hmgn1^-/- mESCs, Hmgn2^-/- mES

SUBMITTER: Gohil R 

PROVIDER: S-EPMC12796744 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature