Decoding innate lymphoid cell heterogeneity and plasticity in colorectal cancer.

ABSTRACT:

Background

In colorectal cancer (CRC), innate lymphoid cells (ILCs) play a vital role in preserving and modulating immune homeostasis within the intestinal environment. However, the origins and diverse functions of ILCs in CRC remain poorly understood, making it difficult to clarify how these cells contribute to disease progression and influence therapeutic efficacy.

Methods

Single-cell RNA sequencing (scRNA-seq) generated an atlas of ILCs from multiple tissues (bone marrow, blood, and intestine), revealing their origins, heterogeneity, and plasticity. Spatial transcriptomics (ST) and immunofluorescence (IF) defined their specific cellular neighbourhoods within the tumour microenvironment.  In vitro co-culture assays were performed to validate the regulatory role of ILC2s in

SUBMITTER: Zhang S 

PROVIDER: S-EPMC12796840 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature