Chiral separation of aromatic amino acids by capillary electrophoresis using sulphated β-cyclodextrin as a single chiral selector: an experimental and computational study.

ABSTRACT: Chiral discrimination of aromatic amino acids is crucial in biochemical, pharmaceutical and analytical sciences, yet a clear and mechanistic understanding of cyclodextrin-mediated enantioseparation in capillary electrophoresis (CE) remains incomplete. In this work, the enantioseparation of tryptophan (TRP), tyrosine (TYR), and phenylalanine (PHE) was studied by capillary electrophoresis (CE) using sulphated β-cyclodextrin (S-β-CD) as a single chiral selector under acidic buffer conditions. Separation parameters, including S-β-CD concentration and background electrolyte (BGE) pH, were systematically investigated. Base line separation of the three analytes was achieved using 1.5 mM S-β-CD in 25 mM phosphate buffer at pH 2.5. In addition to analytical performance, the contribution of this stu

SUBMITTER: Elbashir AA 

PROVIDER: S-EPMC12797201 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature