DNA methylation predicts lung function and pulmonary exacerbation in sputum samples from patients with cystic fibrosis.

ABSTRACT:

Background

Lung disease is variable among patients with cystic fibrosis (CF) and depends on genetic and environmental factors. To better understand the molecular determinants of lung disease variability, we carried out an epigenome-wide association study (EWAS) in sputum samples from patients with CF.

Methods

We profiled 64 sputum samples using Human Methylation EPIC BeadChips and assessed the correlation between DNA methylation levels and four clinical traits: lung function (FEV1_pp), lung function variation (FEV1_pp slope), presence and number of pulmonary exacerbations. Sputum samples were collected at four time points over an 18-month follow-up period. Selected CpG sites were reassessed in independent sputum samples from the same cohort by pyrosequenc

SUBMITTER: Tost J 

PROVIDER: S-EPMC12797401 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature