PRKCSH deficiency promotes an anti-tumor immune microenvironment via UPR activation and M1 macrophage polarization.

ABSTRACT: Lung adenocarcinoma remains one of the most common causes of cancer deaths. The tumor grows by avoiding the immune system and adapting to stress in the endoplasmic reticulum. The IRE1α-XBP1 pathway is a key pathway for cells to sense stress in the endoplasmic reticulum and has a large effect on the immune system. PRKCSH encodes a regulatory subunit of glucosidase II that helps keep the endoplasmic reticulum in balance by modifying how IRE1α works. However, it is unclear how it affects tumor immunity. This study used clinical sample analysis, bioinformatic analysis, CRISPR/Cas9-mediated gene deletion, cytokine profiling, macrophage co-culture, and zebrafish xenograft experiments to investigate the immunological role of PRKCSH. PRKCSH deficiency reduced basal IRE1α phosphorylation but led to

SUBMITTER: Xiyuan G 

PROVIDER: S-EPMC12797428 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature