Recombinant MAM from Faecalibacterium duncaniae exhibits a protective effect in DNBS-induced colitis.

ABSTRACT:

Background

Microbial anti-inflammatory molecule (MAM) is a key effector of the next-generation probiotic Faecalibacterium duncaniae A2-165, a species whose depletion in the gut microbiota is strongly linked to inflammatory bowel disease (IBD) and other conditions. Despite its importance, the direct anti-inflammatory effects of purified MAM have never been evaluated in vitro or in intestinal inflammation models. Prior studies have relied on bacterial supernatants, synthetic peptides, or DNA delivery systems, each with inherent limitations.

Results

In this study, we produced and purified recombinant MAM (R-MAM) under denaturing conditions and, for the first time, demonstrated its direct anti-inflammatory activity in vitro and its protective effects in a colitis murine model. D

SUBMITTER: Vilela Rodrigues T 

PROVIDER: S-EPMC12797504 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature