Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering.

ABSTRACT: Methanol is an attractive one-carbon feedstock for sustainable biomanufacturing because of its abundance, cost-effectiveness, and industrial compatibility. However, its cytotoxicity limits its biotechnological applications in native methylotrophs such as Methylobacterium extorquens AM1. In this study, we developed AM1-derived strains capable of sustained growth under elevated methanol concentrations through adaptive laboratory evolution (ALE). From the evolved population, five representative strains were isolated, exhibiting up to a 1.68-fold increase in specific growth rates compared with those of the wild- type at 2.5% (v/v; 617.93 mM) methanol. Genomic analysis of the evolved strains revealed recurrent mutations in metY (O-acetyl-L-homoserine sulfhydrylase) and kefB (potassium efflux an

SUBMITTER: Lee GM 

PROVIDER: S-EPMC12797532 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature