MiR-338-3p targets WAPL to suppress proliferation and invasion in cervical cancer cells: experimental insights.

ABSTRACT:

Objective

This study aimed to elucidate the role of miR-338-3p in cervical cancer, focusing on its regulatory effect on the WAPL (wings apart-like homolog) gene and the consequent influence on the behavior of cervical carcinoma cells.

Materials and methods

Bioinformatic analysis identified a potential miR-338-3p binding site in the WAPL 3' untranslated region (3'UTR). This interaction was confirmed using a dual-luciferase reporter assay. The effects of miR-338-3p overexpression on the proliferation, apoptosis, cell cycle, and invasion of cervical cancer cells were subsequently evaluated.

Results

WAPL transcript levels were elevated in cervical cancer tissues compared with normal controls (P < 0.05). The GV369/miR-338-3p construct was successfully generated, and qRT-PC

SUBMITTER: Li B 

PROVIDER: S-EPMC12797562 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature