PiRNA DQ690018 increased bone mass by promoting the proliferation and osteogenic differentiation of BMSCs.

ABSTRACT:

Background

The proliferation, migration, and osteogenic differentiation of bone mesenchymal stem cells (BMSCs) play vital roles in maintaining bone mass and metabolism. BMSC base cell therapy has become a competitive treatment option for osteoporosis. PIWI-interacting RNAs (piRNAs) are critical regulators of cellular processes.

Methods

Small RNA sequencing and qPCR were used to detect piRNA expression during BMSC proliferation. CCK-8, clone formation, and EdU assays were used to detect the effect of piRNA DQ690018 on BMSC proliferation. Wound healing and Transwell assays were used to assess the effect of piRNA DQ690018 on the migration of BMSCs. RNA sequencing was performed to identify DQ690018. Alkaline phosphatase and alizarin red staining were used to evaluate the effect

SUBMITTER: Li Y 

PROVIDER: S-EPMC12797675 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature