Eimeria tenella apical membrane 2 overexpression enhances pathogenicity and immunity in chickens.

ABSTRACT:

Background

Eimeria tenella, a highly pathogenic apicomplexan parasite, causes severe avian coccidiosis, threatening global poultry production. Apical membrane antigens (AMAs), conserved proteins in apicomplexan parasites, are critical for host cell invasion, making them promising vaccine targets. In this study, we constructed an the overexpression strain of E. tenella AMA2 (EtAMA2-OE) and evaluated its pathogenicity and immunogenicity.

Methods

Transcriptional profiling of EtAMA2 during infection was conducted using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). A homozygous EtAMA2-OE strain was generated using plasmid transfection, drug selection, and flow cytometry. Pathogenicity was assessed through in vitro sporozoite invasion assays and

SUBMITTER: Wu S 

PROVIDER: S-EPMC12797683 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature