Neutrophil extracellular traps released by CD177⁺ neutrophils aggravated inflammation and neuronal impairment post-SCI.

ABSTRACT: Spinal cord injury (SCI) causes irreversible motor and sensory dysfunction. Initial mechanical injury breaches the blood-spinal cord barrier (BSCB), triggering a rapid influx of neutrophils. However, previous studies on neutrophils after SCI have been relatively limited, the function and heterogeneity of neutrophils need further study. Here, we identified 3 distinct neutrophil subclusters post-SCI using scRNA-seq in SCI mouse models. Additionally, elevated levels of CD177⁺ neutrophils may aggravate inflammation and neuronal impairment based on our data from SCI patients and mice models. Further in vivo and in vitro assays indicate that CD177⁺ neutrophils induce pro-inflammatory polarization of macrophages and microglia via neutrophil extracellular traps (NETs) formati

SUBMITTER: Dai S 

PROVIDER: S-EPMC12797700 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature