Human stem cells loaded with visible and near-infrared fluorescent core-shell polymeric nanoparticles and revealing tropism toward inflammatory stimuli: in vitro evaluation.

ABSTRACT: Theranostic approach based on fluorescent nanoparticles (NPs) internalized by stem cells (SCs) injected in the bloodstream allows for reaching cancer or inflammatory foci with tracking and treating purposes. At the same time, the influence of internalized NPs on SCs properties, namely, their homing, migration ability, and functioning, remains scarcely investigated. Here, H1 human embryonic stem cells (hESCs) labeled with visible (Vis) and near-infrared (NIR) fluorescent NPs are studied in vitro, focusing on the NPs internalization and release efficiency, cytocompatibility, intracellular distribution, as well as the migration ability of hESCs carrying NPs. Polymeric NPs with a polystyrene (PolySt) core and a shell of co-polymer of N-isopropylacrylamide (NIPAM) and acrylamide (AA), [Poly(NIP

SUBMITTER: Bari RZA 

PROVIDER: S-EPMC12797728 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature