Plate-based 10X Genomics-compatible single-cell RNA-sequencing based on Smart-seq3xpress.

ABSTRACT:

Background

10X Genomics (10X) is a leading single-cell RNA-sequencing (scRNA-seq) technology that permits gene expression and T-cell receptor (TCR) repertoire profiling. However, its microfluidics-based approach restricts direct pairing of upstream indexed single-cell sorting with downstream scRNA-seq data and poses challenges for cost-effective profiling of small-scale cell populations.

Results

To address these limitations, we developed a plate-based, 10X-compatible (PB10X) scRNA-seq strategy built on the Smart-seq3xpress (SS3X) principle. PB10X supports indexed sorting using Fluorescence Activated Cell Sorting (FACS) directly into 384-well plates and generates cDNA compatible with any standardized 10X Single Cell 5' library construction kit including the 5' V(D)J and 5' Ge

SUBMITTER: Deserranno K 

PROVIDER: S-EPMC12797766 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature