Single-cell sequencing reveals MCAM⁺ MyCAFs as key pro-angiogenic cells interacting with endothelial cells in solid-type adenoid cystic carcinoma.

ABSTRACT:

Background

Solid-type adenoid cystic carcinoma (ACC) is the most aggressive pathological type with the poorest prognosis. Extensive neovascularization and recruitment of cancer associated fibroblasts (CAFs) are the main characteristics, but the relationship between the two remains unclear.

Methods

Single-cell RNA sequencing was performed to classify and characterize CAFs in ACC. Primary CAFs from ACC were isolated and purified, and the pro-angiogenic capacity of CAFs from solid-type and non-solid-type ACC were compared. Finally, the upstream regulatory mechanisms of pro-angiogenic factor transcription were studied both in vitro and in vivo.

Results

Compared to non-solid-type ACC, CAFs in solid-type ACC are predominantly composed of melanoma cell adhesion molecule (MCA

SUBMITTER: Yuan X 

PROVIDER: S-EPMC12797820 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature