Single-cell transcriptome analysis reveals regulatory programs of prognosis-associated RNA binding proteins during LIHC development.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC), a highly aggressive primary liver cancer, exhibits unclear heterogeneity in RNA-binding protein (RBP) expression dynamics. This study investigates single-cell-level RBP heterogeneity, regulatory roles in HCC progression, and their therapeutic potential.

Methods

Utilizing TCGA-LIHC data, prognosis-associated RBPs were screened by univariate Cox analysis. Tumor clusters stratified via NMF algorithm underwent differential gene expression profiling and functional enrichment (GO/KEGG). Single-cell RNA sequencing dissected RBP heterogeneity in malignant and myeloid-derived cell populations, exploring intercellular interactions. Key RBP gene HDGF was functionally validated through in vitro assays (CCK-8, EdU, Transwell). SpliceSeq analysis

SUBMITTER: Zhang ED 

PROVIDER: S-EPMC12797827 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature