Immunogenicity and plasmid delivery pathways of non-invasive <i>Lactococcus lactis</i>-vectored mucosal DNA vaccination.

ABSTRACT: Mucosal DNA vaccination using a non-invasive Lactococcus lactis (LL) vector has been investigated. However, its immunogenicity and plasmid transfer mechanisms remain largely unknown. In this study, we investigated the intranasal delivery of LL carrying a mammalian enhanced green fluorescent protein (EGFP)-expressing plasmid and the cellular pathways underlying DNA transfer. Intranasally administered LL was primarily localized on the nasal epithelial surfaces, and a smaller fraction penetrated the subepithelial tissues. Intranasal administration of LL-carrying pLEC-EGFP plasmid induces antigen-specific serum IgG and mucosal IgA responses. In vitro co-culture analyses demonstrated that plasmid delivery and expression occurred in phagocytic cell lines but not in epithelial cell

SUBMITTER: Kawashima S 

PROVIDER: S-EPMC12798020 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature